ABSTRACT
Objective To investigate the expression status of A Disintegrin and Metalloproteinase with Thrombospondin motifs 5(ADAMTS5 )and its clinical significance in primary human gastric cancer(GC). Meth-ods ADAMTS5 protein expression in 176 GC tissues and 24 adjacent normal tissues was detected by immunohisto-chemistry(IHC),and its correlation to clinicopathological features and prognosis in patients with gastric cancer was analyzed. Results ADAMTS5 expression was lower in GC tissues compared with adjacent normal tissues. Moreover,low expression of ADAMTS5 was associated with gender,histologic type,degree of differentiation,M stage,TNM stage and vascular invasion,which was also an independent indicator for poor prognosis for GC pa-tients. Conclusions ADAMTS5 expression was lower in GC tissues,which was associated with clinicopathological features and prognosis,suggesting a novel potential prognostic indicator and therapeutic target in GC.
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Objective To investigate the effect of a disintegrin and metalloprotease domain(ADAM8)gene on colon cancer HCT8 cell proliferation and proliferation signal transduction pathways PI3K-Akt-mTOR. Methods Colon cancer HCT8 cells were cultured in vitro,and transfected with ADAM8 overexpression plasmid and RNA interfering plasmid. Cell proliferation were detected by EdU and MTS method assays. PI3K activity was measured by PI3K activity detection kit,Western Blot method was performed to detect the ratio of Akt and p-Akt and expression of mTOR. Results Compared with control group(1.00 ±0.12),the cell proliferation in ADAM8 overexpression group(1.22 ±0.13)was significantly higher (P<0.05)and in RNA interfering group(0.78 ±0.11)was significantly lower while PI3K activity had no significant changes in three groups. After ADAM8 overexpression,and the ratio of p-Akt/Akt and mTOR expression were increased significantly,while reduced significantly after RNA interferered. Conclusion ADAM8 can promote HCT8 cell proliferation through enhancing the phosphorylation of Akt and promoting the expression of mTOR.
ABSTRACT
Objective To explore the type of the rational digestive reconstruction after total gastrectomy for gastric malignancy.Metlhods Three types of digestive reconstruction were performed after total gastrectomy in 121 cases with gastric carcinoma.The operating time,morbidity and mortality,digestive tract symptoms,nutritional status in 1 year after operation were compared.Results There were no signifioant differences among the three procedures in operative morbidity and mortality,postoperative food intake,nutritional status,incidences of diarrhea and dumping syndrome.Roux-en-Y esophajejunostomy and P-type esophajejunostomy had an advantage d anti-esophageal reflux,and are obviously superior to Lahey + Braun anastomosis.Roux-en-Y esophajejunostomy was simpler with shorter operating time and less complication.Condusion Roux-en-Y esophajejunostomy and P-type esophajejunostomy could be recommended as suitable methods for digestive reconstruction after total gastrectomy.